Technical Data

Synthesis of Nicotine

All data collected and verified by the Pharmaceutical Science Department at University California Irvine (UCI).

Proton Nuclear Magnetic Resonance

The Proton Nuclear Magnetic Resonance spectra are shown in Figure 1.



PNMR Spectra with Integration

The Proton Nuclear Magnetic Resonance spectra with integration is shown in Figure 2. Integration provides valuable information regarding the relative number of hydrogen in the molecule. These data results show the relative positions of the hydrogen atoms on the nicotine molecule, and the lack of any impurity (other “peaks”).fig2

Carbon Nuclear Magnetic Resonance

The Carbon Nuclear Magnetic Resonance spectra is shown in Figure 3. This shows the number of carbon atoms in the molecule, and is consistent with Nicotine.


Gas Chromatogram (GC) and its corresponding Mass Spectra

The data shown in Figure 4, where a Gas Chromatogram (GC) and its corresponding Mass Spectra (MS) are displayed, is the most informative data when analyzing for purity. A gas chromatography device separates compounds mainly by their relative volatility (boiling point). Note the one sharp peak in the Gas Chromatogram (left side), and absence of any other peak, which demonstrates the high purity level. The corresponding Mass Spectrum is shown on the right. A mass of 162 is consistent with Nicotine molecular weight (mass). Therefore, we conclude that the compound that gave rise to the sharp peak in the Gas Chromatogram was Nicotine, and we also conclude that its purity level is > 99.5%.


Gas Chromatogram Continued...

Figures 5,6,7,and 8 are the Gas Chromatogram, and in Figures 6,7,8 the baseline was expanded to demonstrate the lack of any other peaks (impurities).





Purity Validation

– TFN® Nicotine is an extremely pure form of nicotine.


– TFN® Nicotine is not derived from tobacco leaf, stem or waste dust.


– The purity of TFN® Nicotine is evidenced mainly by the spectroscopic data, and specifically to the Nuclear Magnetic Resonance data (H1NMR and C13NMR) as well as a more traditional Gas Chromatographic analysis, and a state of the art Mass Spectral analysis performed at the University of California Irvine Organic Chemistry Department.


– The H1NMR data show the connectivity of the hydrogen atoms of the Nicotine molecule. If there are other molecules present, we would definitely see their resonance and they would appear in this spectra.


– Also, in the carbon NMR (C13NMR), where the number of carbons and position on the spectra are consistent with the structure of nicotine, and nothing else appears in the spectral data, are consistent with the ultra-high purity of TFN® Nicotine.


– The GC-MS, where the gas chromatography (GC) is coupled with mass spectrometry (MS), provides for a very sensitive method for analysis of purity.